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lcfB [2018-03-08 16:03:50]
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lcfB [2018-03-08 16:03:50]

long-chain fatty-acid-CoA ligase, involved in surfactin production
locus
BSU10270
pI
5.30
mw
56.41 kDa
protein length
513 aa Sequence Blast
gene length
1539 bp Sequence Blast
function
fatty acid degradation
product
long-chain fatty-acid-CoA ligase
essential
no
ec
6.2.1.3
synonyms
yhfL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]

    • Gene

    Coordinates
    1,100,980 → 1,102,521

    The protein

    Catalyzed reaction/ biological activity

  • ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis [Pubmed|20797616]

    • Protein family

  • ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|5050649605DD782AF10FC0AEEA5F14036EC0197D|YhfT], [protein|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|YngI], [protein|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|LcfA]

    • Structure

  • [PDB|3R44] (from Mycobacterium tuberculosis, 35% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|lcfB]
    description
    [Pubmed|17189250]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|17189250], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]: repression, [Pubmed|17189250], in [regulon|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • induced in the presence of long-chain fatty acids ([protein|search|FadR]) [Pubmed|17189250]
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    Biological materials

    Mutant

  • MGNA-B277 (yhfL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1276 NBRP B. subtilis, Japan]
  • available in [SW|Mohamed Marahiel]'s lab [Pubmed|20797616]
  • BKE10270 (Δ[gene|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|lcfB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCCACCCCTTAAGT, downstream forward: _UP4_TAAAATGAAAAAGCGAAGCG
  • BKK10270 (Δ[gene|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|lcfB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCCACCCCTTAAGT, downstream forward: _UP4_TAAAATGAAAAAGCGAAGCG

    • References

  • 17189250,17919287,20797616